1. Check your work
Sterilizers are somewhat analogous to household dishwashers: one simply loads dishes/instruments into the machine and then starts the cycle. In the case of the sterilizer, the process is supposed to get everything germ-free. But, like our dishwasher comparison, one can’t put in items that are too messy for the machine to handle.
For the sterilizer to do its job, it can be hindered if debris gets in the way. Bcause the debris creates a coating or a covering over oral microorganisms that can allow them to survive the heat, the chemical vapor, or the steam of the sterilizer. There’s evidence that shows that there can still be microorganisms present in debris, even though they’ve been processed through a dental autoclave sterilizer.

2.Handling
Frequently, when I visit dental practices, I see that they have overloaded their sterilizer. They have placed so many instrument pouches or cassettes or combinations thereof together in the sterilizer, that the sterilant cannot do its job of circulating around all the instruments, actually penetrating the wraps... Some states don’t require wrapping of instruments; however CDC recommends that instruments be packaged or wrapped prior to sterilization. If you overload, not everything is going to achieve the benefit of the sterilant, which is usually steam.
How instruments are sterilized is critical. If the manufacturer’s instructions are not followed, it is unlikely that instruments will be properly processed.

3. Be careful
It's not just patients who need to be protected from unsafe instrument handling practices. Carelessly handling instruments can pose a risk to dental team members. Transporting contaminated instruments from treatment room to sterilization should be done safely. Never carry sharp, contaminated instruments by hand.
An easy way to avoid trouble, she recommends, is by using a treatment tray with a locking lid.

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